HPLC Basics Course with Electrochemical Detector Theory and Operation
This 3-day HPLC "back-to-basics" training course is specifically designed for individuals who routinely use analytical HPLC while lacking a solid foundation in the fundamentals. Days 1 and 2 provide the basic principals of HPLC instrumentation and column technology through classroom and hands-on experience, while Day 3 includes specific training on theory and operation of electrochemical detection.
Upcoming Dates:
- May 12-17, 2012
At the end of this three-day course, participants will have a real knowledge of HPLC system operation and maintenance, as well as how to best use these skills in their laboratory. This training will help individuals better understand the use of the Coulochem, CoulArray, and Corona CAD detectors.
Course Overview
- HPLC Basics
- Understanding the HPLC system
- Theory of chromatographic separation
- HPLC column technology
- Mobile phase chemistry and optimization
- Improving separation by controlling variables
- HPLC instrumentation
- Choosing the right detector
- Troubleshooting tips and tricks
- Hands-on operation and routine maintenance
- Electrochemical detectors
- Comparison of electrochemical detectors to other detectors
- Electrochemical theory
- Coulometric vs amperometric
- Optimizing detector selectivity
- Maximizing sensitivity
- Choosing the best cell
- Practical applications
- Hands-on operation
Course Registration
Click here to register.
Instructor Biography
Dr. Bruce Bailey (Lead Instructor) received his Ph.D. in biology from the University of Waterloo, Ontario, Canada, and has co-authored more than 23 publications. Dr. Bailey is currently a senior applications manager and has worked in the applications department of Thermo Scientific Dionex for the past 15 years. He has more than 25 years experience using HPLC with electrochemical detection and helped develop many of the applications currently available. Dr. Bailey has been actively involved with research in neuroscience and free radical biology and has assisted in developing novel application chemistries to study neurochemistry and biomarkers of oxidative stress.
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