SEARCH
ESA
Improving outcomes from discovery to diagnostics ESA
ABOUT USPRODUCTSSERVICESNEWS & EVENTSRESOURCESESA LABORATORIES

Model 542 HPLC Autosampler

Feature-rich and incorporating an integrated column oven, the Model 542 is a cost-effective autosampler for HPLC analysis. This compact-sized unit offers many features found in other more expensive autosamplers. It incorporates three selectable injection modes: full loop, partial loopfill, and microliter pick-up. Sample processing capabilities are provided for sample mixing, dilution, and on-line derivatization.

The integrated column oven provides temperature control up to 60° C and keeps injector to column tubing connection length to a minimum. The fluidic pathway uses biocompatible (PEEK®) components. The quick-change injection valve simplifies change-over and minimizes down time. The standard sample tray capacity is 84 - 1.8 mL sample vials and 3 - 10 mL vial positions for wash and alternate reagents. The sample tray can be cooled (refrigerated) by the addition of optional factory-installed temperature-control capability. Features include:

  • Quick-release bio-compatible injection valve
  • Integrated column oven
  • Integrated sample tray cooling (optional)
  • Injection system providing zero sample loss with limited sample volumes
  • Optional sample tray formats
  • Sample dilution, mixing and derivatization capabilities

Model 542 Specifications

Sample Capacity: Standard tray: 84 vials of 1.8 mL (Std)
and 3 vials of 10 mL (LSV)
Vial Dimensions (including cap): Maximum vial height: 47 mm,
Minimum vial height: 32 mm
Loop Volume: I - 5000 µL (interchangeable)
Dispenser Syringe: 250 µL (Std) or 1000 µL syringe
Vial Detection: Missing vial detected by vial sensor
Headspace Pressure: Built-in compressor
Switching Time Injection Valve: Electrically <100 msec
Piercing Precision Needle: ± 0.6 mm
Wash Solvent: External wash solvent bottle
Bio-compatible Valve: PEEK' injection valve, with quick connect mounting
Wetted Parts in Flow Path: SS316, PTFE, TEFZEL, VESPEL, Glass, Teflon, PEEK
Built-in Column Oven: Temperature Range: ambient +5 to 60 C ± II C
Oven Dimensions: I" W x I" D x 11.811 L (2.5 cm x 2.5 cm x 30 cm)
Communication Port: Serial RS232C
Output: Injection marker, 2 Auxiliary outputs,
Alarm output (Relay & TTL)
Input: Next injection, freeze & stop (TR)
Ambient Environment: 10-40'C, 5-95% relative humidity, non-condensing
Unit Dimensions: 30 cm x 50 cm x 34 cm (11.8" H x 19.7" W x 13.4" D)
Weight: 19 kg (42 lb.), 21 kg (46 lb.) with cooling option
Power Requirements: 115/230 VAC; ± 15%; 50/60 Hz; 200 VAC
Product Certification: CE
PERFORMANCE
Reproducibility:
RSD < 0.3% for full loop injections
 
RSD < 0.5% for partial loop fill injections,
injection volumes > 10 µL
 
RSD < 1.0% for µL pick-up injections,
injection volumes > 10 µL
Carry-Over: < 0.05% with programmable needle wash
PROGRAMMING
Methods: 9 programmable methods with priority sample mode
Injection Modes: Full loop injection, Partial loop fill injection,
µL pick-up injection
Injection Volume: I µL - 5000 µL, with I µL increments
Injections per Vial: Maximum 9 injections
Analysis Time: Maximum 9 hr. 59 min. 59 sec.
Programmable Needle Wash: Wash between injections, between vials, between series
Programmable Timed Events: 4 x ALJX-1 on/off, 4 x AL7X-2 on/off,
Initial oven setpoint, 2 x new oven setpoint
Series:
9 programmable series
SAMPLE TRAY COOLING
Temperature Control: Integrated, Peltier type
Temperature Range/Capacity: 4-15º C, no greater than 20º' C below ambient
Temperature Accuracy: ± 2º C

Specifications subject to change without notice

Printable version
Site map Terms & condition of use Privacy policy ESA Biosciences Trademarks ©1968-2008. Magellan Biosciences Contact us Product Registration Home