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Model 540 HPLC Autosampler

This unique double-needle autosampler is an extremely versatile automatic sample-injection system for HPLC, making it ideal for microdialysis studies. It uses a stainless steel needle to penetrate the septum and then second needle for sample aspiration.

Features include:

  • Three injection modes – flushed loop, partial loop and microliter pick-up for excellent reproducibility and no sample loss when dealing with limited sample availability
  • Automated derivatization and diluting capabilities – flexible formats for sample preparation
  • Peltier sample temperature control – integral heating and cooling of the sample racks
  • Flexible vial format – supports a wide range of sample vial shapes and sizes
  • Automatic vial sensor system – detects vial presence and size

Model 540 Specifications

Sample Capacity: 96 1.5 mL vials, 72 4.0 mL vials, 160 0.5 mL vials, 32 10 mL vials; Sample rack consists of 8 segments which can include any combination of formats
Injection Volume: 1 - 1000 µL
Injections/Vial: Maximum of 9
Analysis Time: Maximum 9 hr 59 min. 59 sec.
Syringe: 100 µl, 250 µL, 500 µL, or 1000 µL
Needle Wash: Programmable, 250 mL wash solvent bottle built in
Reproducibility: Flushed Loop - <0.3% RSD; Partial Loop - <0.5% RSD; µL Pick-up - <1.0% RSD
Memory Effect: <0.01% with programmable needle wash
Power Requirements: 110 V or 220 V selectable on rear panel
Communication: Outputs – inject marker, vial marker, labeled vial marker, stop I/O (all relay & TTL), BCD (TTL), 4 auxiliary (relay), 4 programmable outputs (relay), 4 bit time base (TTL)
Inputs – next injection marker, next vial marker, stop I/O, 4 programmable inputs (all TTL)
Dimensions: 11 in. (W) x 21.3 in. (D) x 17.3 in (H)
(280 mm x 540 mm x 440 mm)
Weight: 22 kg (48 lbs.)
Options
Sample Temperature Control: Built-in Peltier control 4-40° C (factory installed)
Integrated Stream Switching: Two optional high-pressure 6-port valves (factory installed)
Micro Version: Special injection valve and needle; injection range: 0.1 µL to 2.5 µL; comes with sample temperature control 
LSV Tray: 72 positions for 4.0 mL vials
Super LSV Tray: 32 positions for 10 mL vials

Specifications subject to change without notice

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