Quantitative bioanalysis
ESA offers the following bioanalytical services:
8-Hydroxy-2’-deoxyguanosine (8-OH-2’dG or 8-Oxo 2’dG) Using ESA's proprietary technology, we offer analysis of this DNA adduct marker of oxidative stress from urine, plasma, and hydrolyzed DNA.
Amino Acids The study of low level amino acids in microdialysis perfusates is accomplished using pre-column derivitization with o-phthaldialdehyde (OPA)/2-mercaptoethanol (BME) prior to detection with ESA's Coulochem® III electrochemical detector.
Catecholamines Various medical conditions have been associated with increased levels of norepinephrine, epinephrine, and dopamine. We use ESA’s CoulArray® detector-based system to accurately measure catecholamines in plasma or urine samples.
CoEnzyme Q9 and Q10 These important antioxidants can be easily measured in both oxidized and reduced forms to monitor the efficacy of uptake and the antioxidant effectiveness in bioequivalency studies.
Monoamines Various columns, cells, and mobile phases are available for contract studies to measure a variety of monoamines and metabolites from tissue samples, microdialysis perfusates, and cell culture samples.
Phytoestrogens These plant-derived substances have estrogenic activity in animals. There is a great deal of interest in potential benefits and possible adverse effects from phytoestrogens. Isoflavones (daidzein and genistein), the metabolite equol, and the lignan enterolactone are all measured from serum.
Tea Catechins Green tea in particular is abundant in polyphenols – specifically, a group of flavanols commonly known as catechins. Recent studies suggest that tea consumption may have beneficial effects on carcinogenesis. Levels of epicatechin, epicatechin gallate, epigallocatechin, and epigallocatechin gallate are measured simultaneously using an ESA’s CoulArray® system.
Total Thiols Thiols are powerful reducing agents capable of antioxidant effects in vivo. They exist in three forms: the free thiol (RSH), the homodisulfide (RSSR), and the heterodisulfide (RSSR’). Following extraction, total thiols are analyzed by reducing the disulfides to the free thiol form followed by derivitization and analysis.
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